a. water used for the preparation of the

a.        Non-microbiological aspects include the following.

1.       Quality of the medium: medium with highquality has to be used. Quality of the water used for the preparation of theculture medium is important. After preparing the medium osmolarity has to bechecked. Chemical analysis for the concentration of Na2+, k+,ca2+, glucose etc.., can be determined.

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Biological examination suchas survival test for sperm/ growth of mouse embryos has to be carried out. Torule out embryo toxicity, Bio controls are strongly recommended if newmaterials are introduced. Destination per batch of the medium should be recordedin a log book. 2.       Access to IVF laboratory: Laboratoryshould be accessible only to the staff and it should be locked outside workinghours. Cryo-can with embryos and semen has to be secured with lock.

Incubatorsshould be kept locked when cleaning is done. 3.       Emergency systems: Lab should be withprovision for continuity of the electricity and CO2 supply. Automatic switchingto reserve bottles is also available in some lab settings. All the incubatorsshould be fitted with alarm. Level of liquid nitrogen should be checkedperiodically by a system of initiating or by electronic monitoring.

4.       Regulations to prevent mix-up of gametesand embryos: every centre has its own protocol and rules. First thing inIVF lab maintenance is absolute essential of good labelling in the tubes anddishes with the name of the male and female partner. Aspirates from differentpatients have to be processed one by one.

Good communication between the clinicianand the embryologist is necessary for carrying out punctures during OPU, for ETtimes etc.., In case of embryo transfer name of the couple has to be clearlyclarified. In case of frozen embryo transfers double verification isrecommended. Finally it is also important to employ staffs who work accurately.