In determine these 24 genes in different terms

In three regions of
root tips we first  analyze the genes accelerated in response
to exogenous ABA .
We detected 1444, 838, and 855 genes that are expressed differentially  before and after ABA treatment in Tip,of LRH,
and SRH. Mostly, 1070, 353, and 477 genes decoded
 specifically in root
Tip, LRH, and SRH, respectively.through studies we analyse
that genes mostly expressed in all areas  indicated that most of the ABA upregulated
genes are in the apex , mostly in LRH, and lesser in SRH . results suggested that the rice root
tip zones detect and respond
to ABA.

I order To understand
genes expression  in the LRH  the whole biological processes taken place in
the LRH zone by ABA treatment,experiments are  performed (GO) for  enrichment analysis of gene ontology  of the 353
significantly ;we determine these 24 genes in different terms . The
first category involved in different stress responses, such as “response
to oxidative stress,” or 
to chemical stimulus,” “response  to  stimulus,” 
and  “response to stress.”
The second category  involved in
“metabolic pathways,” such as
“glycine mediated process,” “metabolic process
of small molecule ,” “primary metabolic process,” “cellular
amino acid and derivative metabolic process,”
“lipid metabolic process,” “cellular
carbohydrate metabolic process,” and
“polysaccharide metabolic process.” Rather, 19 genes specifically responding to
ABA stress in LRH Nine of the 19 stress-responsive genes  encoded peroxidases , production of reactive
oxygen species have important roles in growth of root hairs in response to ABA

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Because of the
importance of auxin in root hair elongation to understand whether these
differentially expressed genes are related to ABA-regulated root hair
elongation, we checked whether auxin affects the 353 genes that are
differentially expressed in the LRH by comparing the genes to the auxin-
regulated genes in the RiceXpro database (Sato et al.,2013). As shown in Figure
6D, among the 192 ABA upregulated genes, 
57 (  29.7%) were also upregulated
by auxin (Table 1), and 56  of the 161
ABA downregulated genes were also repressed by auxin (Table 2). This suggests
that auxin plays an important  role in
ABA-promoted rice root hair elongation, and the 113 genes co-regulated by both
ABA and auxin in the LRH zone may function directly in regulating root hair
elongation. We found that OsPIN9 and OsPP2C 59 are upregulated, further
supporting the importance of polar auxin transport in root hair elongation.

auxin (2000 bp upstream
of the start codon) for the RHE sequence 
“WHHDTGNNN(N)KCACGWH” (where W            A/T,
H A/T/C, 
D        G/T/A,  K        G/T,  and  N    A/T/C/G), as previously described (Won
et  al.,2009).  We 
found  69  RHEs in 51 genes, with 13 genes carrying two
or more RHEs (Table 3 and Supplementary Table 5). Because we lack root hair-
specific gene expression data, we predicted whether these genes are
specifically expressed in epidermal cells by searching the rice microarray
expression database (RiceXPro) (Supplementary Table 5). These results indicated
that 35 out of the 51 genes containing the RHE are highly expressed in
epidermal cells in rice root tips. 


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