The intent of this experiment was to analyze the rate of phagocytosis of the specific protist known as Tetrahymena vorax. This certain sort of protist provenders at a normal rate when conditions are normal. We began the experiment of understanding how Tetrahymena provenders at normal temperatures. Making so. we allowed the Tetrahymena to feed at specific clip bounds and so killed the protist with gluderaldheyde to observe how much the protist gained. We so counted how many nutrient vacuoles were filled with India ink that was included in the environment of the Tetrahymena while the clip was clicking.
We so constructed a similar experiment with different conditions to compare if the rate of phagocytosis was affected by the alteration in conditions. Comparing the two environments. we found that so the rate of Phagocytosis was affected by a alteration in environmental conditions. Introduction Tetrahymeha Vorax consist of fresh water protists that normally are egg-shaped molded if non about egg shaped. This being belongs to a specific group known as the ciliophorans ( Cambell ) . They use specialised eating constructions such as the Buccal Cavity to consume their nutrient into their unwritten pit ( Shefferly. 2010 ) .
The Tetrahymena provender at different rates over clip and can alter with environmental variables. The nutrient that is ingested into the Tetrahymena is contained in the nutrient vacuoles inside of the being. After the Tetrahymena has completed it’s eating and the nutrient vacuoles are filled. the digestion procedure so occurs. The digestion procedure has a certain type of cell organ to ask for enzymes to interrupt down the nutrient atoms known as lysosomes. This so leads into the elimination of waste for the Tetrahymena.
Any nutrient atoms that were left undigested are so removed from the cell at a specific point inside of the cell called the cytoproct ( Shefferly. 2010 ) . Sing this procedure that the protist undergoes. we predicted that Cold temperatures could impact beings by decelerating down their metamorphosis. Reasoning behind this is that if any type of life being is placed in a much colder environment however a different environment instead than normal. its working organic structure procedures. such as feeding behaviour. will execute at a much slower rate ( Curds ) .
We started out with analyzing the Tetrahymena eating in normal conditions. India ink. which is the stuff the Tetrahymena Federal on. was added to the Tetrahymena environment. We increasingly timed the Tetrahymena as it performed phagocytosis. After the clip was up. we examined how many nutrient vacuoles were filled with the India ink to demo how much the Tetrahymena had captured in the clip bound given under normal conditions. These consequences of this experiment acted as a control for our following experiment we planned on acting.
The following experiment consisted of the same type of processs of our control experiment but we changed the environment to a much colder environment by puting the Tetrahymena in an ice bath to see if the cold temperature affected the rate of phagocytosis. Before the experiment we hypothesized that a lessening in temperature will impact the Tetrahymena’s rate of phagocytosis. We besides made anticipations before the experiment in the context that if we placed the Tetrahymena in a colder environment. so the rate of phagocytosis would diminish. Materials and Methods
In the first experiment we were proving the rate of phagocytosis of the Tetrahymena. which would in bend be our control experiment. We foremost started off garnering 6 microfuge tubings for the experiment. We labeled them by the clip bound each trial tubing would stand for such as T0. T2. T5. T10. T20. and T30. We so inserted a chemical known as gluteraldehyde by a micropipette in the sum of 10ul into each trial tubing that was labeled. Following. we took the Tetrahymena that was provided to us from being grown in normal conditions in the sum of 3ml and 30 ul of India ink into a specfic trial tubing.
We made certain to get down clocking right when the mixtures were combined. Basically the T0 sample showed us that it was the base for comparing the different clip bounds because the clip bound for that trial tubing was under a minute. As the clip bound came to an terminal for that trial tubing. we took a sample of about 20 ul of the ink and cell mixture and inserted it into the labelled T0 microfuge tubing. which contains gluteraldehyde. We besides made certain to shut the top so the suspension was non able flight. We proceeded making that process for every other trial tubing.
We timed the other samples at 2 proceedingss. 5 proceedingss. 10 proceedingss. 20 proceedingss. and 30 proceedingss. Following. we set aside the 20 ul sample for after all of our timing was done to compare all samples. As we continued to wait for the timing of our samples we started to detect the Tetrahymena and how they were feeding on the India ink. By making this. we placed populating Tetrahymena and India ink onto a slide to analyze under the microscope. We were able to detect how the Tetrahymena cells were executing phagocytosis and doing motion.
We were besides able to detect and pick out certain constructions of the Tetrahymena such as the cytostome. and nutrient vacuoles. We were able to finish all of the timed samples of the Tetrahymena and India ink and we proceeded to analyze each single sample under the microscope. We used a compound microscope to detect and garner informations on the samples. We placed a individual bead of the suspension of each microfuge tubing on a slide to analyze. We foremost moved the magnification lens to 10-40x and started to analyze the nutrient vacuoles of the protist.
We counted the figure of nutrient vacuoles that contained a black colour. which was the India ink. Finally. after we gathered all of our informations we placed our consequences in a graph. In the 2nd experiment of the lab. we about performed the control experiment verbatim but made a few alterations to it. Alternatively of holding the Tetrahymena tested in normal conditions. we tested the protist in a much colder environment by a sample into an ice bath and clocking it while it sat in the cold environment. Another alteration we made compared to the control experiment was that we merely used one sample alternatively of utilizing all of the timed samples.
We specifically used microfuge tubing T10. which we timed for 10 proceedingss. Our variables were different every bit good in this experiment by doing clip our independent variable. the figure of nutrient vacuoles filled with India ink our dependent variable. and our first experiment our control. which was Tetrahymena feeding in normal temperature. By executing these two experiments we were able to uncover how colder temperature eating of Tetrahymena resulted in different informations than that of in normal temperatures.